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Amersham Pharmacia Biotech Ltd version 2 microarray hybridization buffer
Version 2 Microarray Hybridization Buffer, supplied by Amersham Pharmacia Biotech Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/version+2+hybridization+buffer/version+2+microarray+hybridization+buffer/pm17470200-44-14-19
Average 90 stars, based on 1 article reviews
version 2 microarray hybridization buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: Dexamethasone and tumor necrosis factor-alpha act together to induce the cellular inhibitor of apoptosis-2 gene and prevent apoptosis in a variety of cell types.
Article Snippet: Concentrated probes were added to hybridization solution (1 Version 2 Hybridization buffer (Amersham Pharmacia Biotech), 50% formamide, 75 ng/ l poly(A)80 and 75 ng/ l human CoT-1 DNA), denatured by boiling, and applied to isopropanol-washed, denatured microarrays under a 22 60-mm glass coverslip.

Purification:

Article Title: Inverse gene expression patterns for macrophage activating hepatotoxicants and peroxisome proliferators in rat liver.
Article Snippet: Macrophage activation contributes to adverse effects produced by a number of hepatotoxic compounds.. Transcriptional profiles elicited by two macrophage activators, LPS and zymosan A, were compared to those produced by 100 paradigm compounds (mostly hepatotoxicants) using cDNA microarrays.. Several hepatotoxicants previously reported to activate liver macrophages produced transcriptional responses similar to LPS and zymosan, and these were used to construct a gene signature profile for macrophage activators in the liver.

Article Title: Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B
Article Snippet: Total RNA was extracted from cells by using the RNeasy kit (Qiagen, Chatsworth, CA), treated with DNase, and repurified. .. One round of T7 polymerase-based linear RNA amplification was performed by reverse transcription of RNA with a T7 promoter oligo(dT) primer and Cy3-dCTP-labeled fluorescent cDNA probes synthesized from the amplified RNA as described ( 10 ), except that to degrade the amplified RNA template, RNaseH (10 units) and RNaseA (10 units) were added and incubated at 37°C for 20 min. Then the probes were purified with a PCR purification kit (Qiagen), vacuum-dried, and resuspended in 50 μl of hybridization buffer [Version 2 hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ) with 50% formamide] containing human Cot1 DNA (Life Technologies). .. The cDNAs printed on the microarrays were from the IMAGE consortium (Integrated Molecular Analysis of Genome and their Expression) and Incyte libraries.

Polymerase Chain Reaction:

Article Title: Inverse gene expression patterns for macrophage activating hepatotoxicants and peroxisome proliferators in rat liver.
Article Snippet: Macrophage activation contributes to adverse effects produced by a number of hepatotoxic compounds.. Transcriptional profiles elicited by two macrophage activators, LPS and zymosan A, were compared to those produced by 100 paradigm compounds (mostly hepatotoxicants) using cDNA microarrays.. Several hepatotoxicants previously reported to activate liver macrophages produced transcriptional responses similar to LPS and zymosan, and these were used to construct a gene signature profile for macrophage activators in the liver.

Article Title: Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B
Article Snippet: Total RNA was extracted from cells by using the RNeasy kit (Qiagen, Chatsworth, CA), treated with DNase, and repurified. .. One round of T7 polymerase-based linear RNA amplification was performed by reverse transcription of RNA with a T7 promoter oligo(dT) primer and Cy3-dCTP-labeled fluorescent cDNA probes synthesized from the amplified RNA as described ( 10 ), except that to degrade the amplified RNA template, RNaseH (10 units) and RNaseA (10 units) were added and incubated at 37°C for 20 min. Then the probes were purified with a PCR purification kit (Qiagen), vacuum-dried, and resuspended in 50 μl of hybridization buffer [Version 2 hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ) with 50% formamide] containing human Cot1 DNA (Life Technologies). .. The cDNAs printed on the microarrays were from the IMAGE consortium (Integrated Molecular Analysis of Genome and their Expression) and Incyte libraries.

Hybridization:

Article Title: Inverse gene expression patterns for macrophage activating hepatotoxicants and peroxisome proliferators in rat liver.
Article Snippet: Macrophage activation contributes to adverse effects produced by a number of hepatotoxic compounds.. Transcriptional profiles elicited by two macrophage activators, LPS and zymosan A, were compared to those produced by 100 paradigm compounds (mostly hepatotoxicants) using cDNA microarrays.. Several hepatotoxicants previously reported to activate liver macrophages produced transcriptional responses similar to LPS and zymosan, and these were used to construct a gene signature profile for macrophage activators in the liver.

Article Title: Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B
Article Snippet: Total RNA was extracted from cells by using the RNeasy kit (Qiagen, Chatsworth, CA), treated with DNase, and repurified. .. One round of T7 polymerase-based linear RNA amplification was performed by reverse transcription of RNA with a T7 promoter oligo(dT) primer and Cy3-dCTP-labeled fluorescent cDNA probes synthesized from the amplified RNA as described ( 10 ), except that to degrade the amplified RNA template, RNaseH (10 units) and RNaseA (10 units) were added and incubated at 37°C for 20 min. Then the probes were purified with a PCR purification kit (Qiagen), vacuum-dried, and resuspended in 50 μl of hybridization buffer [Version 2 hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ) with 50% formamide] containing human Cot1 DNA (Life Technologies). .. The cDNAs printed on the microarrays were from the IMAGE consortium (Integrated Molecular Analysis of Genome and their Expression) and Incyte libraries.

Amplification:

Article Title: Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B
Article Snippet: Total RNA was extracted from cells by using the RNeasy kit (Qiagen, Chatsworth, CA), treated with DNase, and repurified. .. One round of T7 polymerase-based linear RNA amplification was performed by reverse transcription of RNA with a T7 promoter oligo(dT) primer and Cy3-dCTP-labeled fluorescent cDNA probes synthesized from the amplified RNA as described ( 10 ), except that to degrade the amplified RNA template, RNaseH (10 units) and RNaseA (10 units) were added and incubated at 37°C for 20 min. Then the probes were purified with a PCR purification kit (Qiagen), vacuum-dried, and resuspended in 50 μl of hybridization buffer [Version 2 hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ) with 50% formamide] containing human Cot1 DNA (Life Technologies). .. The cDNAs printed on the microarrays were from the IMAGE consortium (Integrated Molecular Analysis of Genome and their Expression) and Incyte libraries.

Synthesized:

Article Title: Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B
Article Snippet: Total RNA was extracted from cells by using the RNeasy kit (Qiagen, Chatsworth, CA), treated with DNase, and repurified. .. One round of T7 polymerase-based linear RNA amplification was performed by reverse transcription of RNA with a T7 promoter oligo(dT) primer and Cy3-dCTP-labeled fluorescent cDNA probes synthesized from the amplified RNA as described ( 10 ), except that to degrade the amplified RNA template, RNaseH (10 units) and RNaseA (10 units) were added and incubated at 37°C for 20 min. Then the probes were purified with a PCR purification kit (Qiagen), vacuum-dried, and resuspended in 50 μl of hybridization buffer [Version 2 hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ) with 50% formamide] containing human Cot1 DNA (Life Technologies). .. The cDNAs printed on the microarrays were from the IMAGE consortium (Integrated Molecular Analysis of Genome and their Expression) and Incyte libraries.

Incubation:

Article Title: Global modulation of cellular transcription by human cytomegalovirus is initiated by viral glycoprotein B
Article Snippet: Total RNA was extracted from cells by using the RNeasy kit (Qiagen, Chatsworth, CA), treated with DNase, and repurified. .. One round of T7 polymerase-based linear RNA amplification was performed by reverse transcription of RNA with a T7 promoter oligo(dT) primer and Cy3-dCTP-labeled fluorescent cDNA probes synthesized from the amplified RNA as described ( 10 ), except that to degrade the amplified RNA template, RNaseH (10 units) and RNaseA (10 units) were added and incubated at 37°C for 20 min. Then the probes were purified with a PCR purification kit (Qiagen), vacuum-dried, and resuspended in 50 μl of hybridization buffer [Version 2 hybridization buffer (Amersham Pharmacia Biotech, Piscataway, NJ) with 50% formamide] containing human Cot1 DNA (Life Technologies). .. The cDNAs printed on the microarrays were from the IMAGE consortium (Integrated Molecular Analysis of Genome and their Expression) and Incyte libraries.



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